Table 1 Carbohydrate-active enzymes identified in the metasecretome of a wheat straw degrading compost-derived community

Proteins unique to biotin-labelled fraction

 c134278_g1_i1_1

CBM2-CBM60-GH10

GH

Beta-1,4-xylanase

BAD88441

0

86

73

Pseudomonas

Bacteria

 c155626_g1_i1_1

CE10

CE

Esterase

WP_051322721

4E-152

77

100

Luteimonas

Bacteria

 c155626_g1_i1_4

GH51

GH

Alpha-N-arabinofuranosidase

WP_024870432

0

80

100

Pseudoxanthomonas

Bacteria

 c180274_g1_i1_4

GH5

GH

Endoglucanase

AIF91557

0

61

83

Alteromonadaceae

Bacteria

 c180864_g1_i1_2

CBM2-CBM22-GH10

GH

Endo-1,4-beta-xylanase

WP_007643754

0

71

99

Cellvibrio

Bacteria

 c189421_g1_i1_1

GH6

GH

Cellobiohydrolase

WP_013117127

0

95

92

Cellulomonas

Bacteria

 c190646_g3_i1_5

CBM2-CBM10-GH6

GH

Cellobiohydrolase

ACE85978

0

67

100

Cellvibrio

Bacteria

 c190817_g1_i1_7

CBM44

CBM

Hypothetical protein

WP_052773020

0

65

90

Luteimonas

Bacteria

 c192149_g1_i1_4

CE10

CE

Peptidase S9

WP_027072364

0

79

91

Luteimonas

Bacteria

 c204578_g3_i1_1

CBM2-CBM22

CBM

Endo-1,4-beta-xylanase

WP_007643754

0

84

98

Cellvibrio

Bacteria

 c207114_g1_i1_5

GH38

GH

Hypothetical protein

WP_051602586

5E-111

35

85

Hyphomonas

Bacteria

 c207852_g16_i3_8

CE10

CE

Hypothetical protein

WP_027072315

0

84

95

Luteimonas

Bacteria

 c208411_g1_i3_1

CBM61

CBM

Subtilisin-like proprotein

WP_014780843

1E-18

37

25

Aequorivita

Bacteria

 c208914_g1_i1_3

CE10

CE

Unknown

CDW81391

3E-42

64

98

Stylonychia

Eukaryota

 c209736_g2_i3_67

GT4

GT

Hypothetical protein

WP_005674657

1E-115

49

99

Lautropia

Bacteria

 c209807_g2_i1_6

SLH

SLH

S-layer protein

WP_013176560

2E-166

37

100

Truepera

Bacteria

 c211555_g1_i1_2

GH11-CBM60

GH

1,4-beta-xylanase

WP_049629015

0

81

100

Cellvibrio

Bacteria

 c27111_g1_i1_1

CBM2-CBM2

CBM

Hypothetical protein

ESQ13017

7E-111

39

99

Uncultured desulfofustis

Bacteria

 c344648_g1_i1_2

CBM50

CBM

Peptidoglycan-binding protein

WP_027071529

0

88

99

Luteimonas

Bacteria

 c345827_g1_i1_2

CE15

CE

Hypothetical protein

WP_012238067

0

68

83

Sorangium

Bacteria

 c528853_g1_i1_2

CBM44

CBM

CARDB domain-containing protein

AEV33755

4E-52

34

51

Owenweeksia

Bacteria

 c711977_g1_i1_3

SLH

SLH

Hypothetical protein

WP_009455020

1E-14

31

29

Fischerella

Bacteria

 c724886_g1_i1_1

CBM2-GH5

GH

Endo-1,4-beta-D-glucanase

ACY24859

0

81

100

Uncultured microorganism

 c80983_g1_i1_2

CBM2-CBM60-CE1

CE

Hypothetical protein

WP_051234546

0

74

99

Marinimicrobium

Bacteria

 c34457_g1_i1_2

AA2

AA

Catalase/peroxidase HPI

WP_036397899.1

2E-78

1

0.9

Mycobacterium

Bacteria

 c210210_g2_i1_1

GH109

GH

gfo/Idh/MocA family oxidoreductase

WP_006979342.1

9E-151

0.99

0.52

Chthoniobacter

Bacteria

 c350217_g1_i1_3

AA6

AA

NADPH-dependent FMN reductase

WP_027072629.1

3E-100

1

0.8

Chthoniobacter

Bacteria

 c180629_g1_i1_1

GH25

GH

glycosyl hydrolase family 25 protein

XP_001008527.1

6E-64

0.98

0.5

Tetrahymena

Eukaryota

 c190646_g2_i1_3

CBM2

CBM

Cellobiohydrolase

WP_007642349.1

0

1

0.74

Cellvibrio

Bacteria

 c208441_g1_i1_2

GH3

GH

1,4-beta-D-glucan glucohydrolase

WP_027070958.1

0

0.97

0.82

Luteimonas

Bacteria

 c151435_g1_i1_2

GH5

GH

Endoglucanase

WP_084618390.1

0

1

0.74

Cellvibrio

Bacteria

 c199479_g2_i1_4

CBM2

CBM

DUF1592 domain-containing protein

WP_007644728.1

0

1

0.78

Cellvibrio

Bacteria

 c207123_g9_i1_7

CE1

CE

S9 family peptidase

WP_043740359.1

0

0.95

0.87

Luteimonas

Bacteria

 c349698_g1_i1_1

GH9

GH

Glycoside hydrolase

WP_041523229.1

0

1

0.7

Gilvimarinus

Bacteria

 c203693_g1_i1_7

CBM4

CBM

Cellulose 1,4-beta-cellobiosidase

WP_085113009.1

7E-19

0.39

0.26

Thermoanaerobacterium

Bacteria

 c225675_g1_i1_1

CBM44

CBM

T9SS C-terminal target domain-containing protein

WP_041627631

2E-52

32

59

Owenweeksia

Bacteria

Proteins unique to supernatant fraction

 c159637_g1_i1_5

DOCKERIN

Dockerin

n.d

 c205510_g2_i1_1

GH74

GH

T9SS C-terminal target domain-containing protein

WP_027376910

7E-41

55

100

Chryseobacterium

Bacteria

 c38599_g1_i1_1

CBM37

CBM

Hypothetical protein

WP_035755077.1

2E-142

1

0.55

Flavobacterium

Bacteria

 c203621_g1_i1_2

CBM6

CBM

T9SS C-terminal target domain-containing protein

WP_040481137.1

1E-86

0.32

0.5

Mariniradius

Bacteria

 c185673_g1_i1_1

AA6

AA

NADPH-dependent oxidoreductase

WP_046482965.1

9E-109

0.99

0.81

Pseudomonas

Bacteria

 c208949_g1_i1_1

PL9-PL9

PL

Nitrous oxidase accessory protein

WP_014202187

3E-19

25

30

Owenweeksia

Bacteria

Proteins common between biotin-labelled and supernatant fractions

 c155243_g1_i1_4

GH38

GH

TonB-dependent receptor

WP_052633156

0

79

99

Pseudoxanthomonas

Bacteria

 c186013_g1_i1_1

GH6

GH

Cellobiohydrolase

WP_027328555

0

77

84

Marinimicrobium

Bacteria

 c194919_g2_i1_5

CBM2

CBM

Carbohydrate-binding protein

WP_007644728

0

77

99

Cellvibrio

Bacteria

 c208473_g1_i1_1

CE8-CBM37

CBM

T9SS C-terminal target domain-containing protein

WP_028522307

4E-74

55

49

Runella

Bacteria

 c194919_g2_i1_4

CBM2

CBM

YceI family protein

WP_087469052.1

0

0.51

1

Cellvibrio

Bacteria

 c209441_g2_i1_1

AA7

AA

FAD-binding protein

WP_053231510.1

6E-164

0.93

0.51

Sandaracinus

Bacteria

 c711379_g1_i1_1

GH74

GH

T9SS C-terminal target domain-containing protein

WP_084016764.1

0

1

0.94

Moheibacter

Bacteria

 c531189_g1_i1_2

CE10

CE

Hypothetical protein

PCJ83084.1

5E-116

1

0.39

Flavobacteriales

Bacteria

 c205510_g1_i1_1

GH74

GH

T9SS C-terminal target domain-containing protein

WP_084016764.1

0

1

0.83

Moheibacter

Bacteria

 c63252_g1_i1_3

GH48

GH

Exoglucanase

WP_013118318.1

0

0.93

0.93

Cellulomonas

Bacteria

 c349698_g1_i1_5

CE1

CE

Feruloyl esterase

WP_072812830.1

2E-67

0.88

0.43

Fibrobacter

Bacteria

 c540340_g1_i1_1

CBM44

CBM

T9SS C-terminal target domain-containing protein

WP_070137948.1

1E-42

0.56

0.6

Crocinitomix

Bacteria

 c190535_g1_i1_1

SLH

SLH

Hypothetical protein

OGI23348.1

2E-140

1

0.39

n.d

Bacteria

 c600504_g1_i1_1

CE8

CE

n.d