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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: The impact of silicon on cell wall composition and enzymatic saccharification of Brachypodium distachyon

Fig. 3

Characterization of the pectin-rich fractions in wild-type (WT) and Bdlsi1-1 plants. Plants were harvested at the ripening stage of growth and at maturity and subdivided as previously described. a Comprehensive microarray polymer profiling (CoMPP) showing the relative abundance of polysaccharide epitopes in the pectin-enriched fraction extracted using 1,2-diaminocyclohexanetetraacetic acid (CDTA). The average signal intensities (n ≥ 2) are presented as a heatmap in which the white colour corresponds to the lowest intensity and red to the highest. The antibody names and their corresponding epitopes are indicated on the Y-axis. Antibodies that did not show signal for all the samples are not included in the heatmap, but listed in Additional file 1: Table S1. The degree of methylesterification of GalA (%) was quantified in the wild type and the Bdlsi1-1 mutant at the ripening stage of growth (b) and at maturity (c). Data represent the mean ± SD, n ≥ 6. All statistical comparisons were made using one-way ANOVA followed by Tukey’s HDS post hoc test, asterisks indicate statistical differences between the wild type and the mutant (**p ≤ 0.01; ***p ≤ 0.001). HG homogalacturonan, RG-I rhamnogalacturonan-I, MLG mixed-linkage glucan, AX arabinoxylan, AGP arabinogalactan protein, GlcA glucuronic acid, FA ferulic acid, HC hemicelluloses

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