Fig. 1

The native PAGE and zymogram analysis of all chitinases purified from C. meiyuanensis SYBC-H1 cells using chitinase–glycogen complex precipitation method. The amount of protein applied to the gel is 10 μg. The gel slice on the left is the coomassie stained one and that on the right is the zymogram. Five proteins [I, II, III (CmChi1), IV and V] with chitinase activity were purified using chitinase–glycogen complex precipitation followed by autodigestion of the complex