Table 1 Oligonucleotide primers used for PCR
Primer | Sequence (5ʹ-3ʹ) | Design basis | Position in Cmchi1 gene (5ʹ-3ʹ) |
|---|---|---|---|
Cmchi1PF | GCTGCCACCCCGACACCGGTTTCGGCTACC | Peptide fragments 1 | 253–282 |
Cmchi1PR | TGCGTTCTTCAGCACCTTGTAGTCCTC | Peptide fragments 2 | 1744–1770 |
ARB1 | GGCCACGCGTCGACTAGTACNNNNNNNNNNGATAT | Arbitrary primer for TAIL-PCR | None |
ARB2 | GGCCACGCGTCGACTAGTACNNNNNNNNNNACGCC | Arbitrary primer for TAIL-PCR | None |
ARB3 | GGCCACGCGTCGACTAGTAC | Arbitrary primer for TAIL-PCR | None |
F-SP1 | GGCGCCGCTCGCGGCACCTAC | Specific primer for TAIL-PCR | 1711–1731 |
F-SP2 | GGTCGTGGCTGGACTGGCGTGCCG | Specific primer for TAIL-PCR | 1654–1677 |
R-SP1 | GTCGTCGCCACCCCGACACCG | Specific primer for TAIL-PCR | 292–312 |
R-SP2 | CGGTGTCGGGGTGGCGACGAC | Specific primer for TAIL-PCR | 344–366 |
Cmchi1-F | CATGCCATGGATGTCGCAAATCAATCGCTTC | Forward primer for CmChi1 | 1–21 |
Cmchi1-R | CCGCTCGAGTTACTTGTTCATGTTGCCCATG | Reverse primer for CmChi1 | 1950–1977 |