Molecular characterization of a novel chitinase CmChi1 from Chitinolyticbacter meiyuanensis SYBC-H1 and its use in N-acetyl-d-glucosamine production

Table 2 Purification of recombinant CmChi1 by different methods

Purification method	Total activity (U)	Total protein (mg)	Specific activity (U/mg)^a	Purification (fold)	Recovery yield (%)
Crude enzyme	198.6	521.1	0.4	0	100
Ni–NTA resin	139.6	12.3	11.4	29.9	70.3
Chitinase–glycogen complex precipitation	176.8	11.6	15.3	40.2	89.0

^aEnzymatic activity was measured in 50 mM citrate buffer (pH 5.2) at 50 °C for 30 min using 1% (w/v) CC as the substrate

ISSN: 2731-3654