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Table 5 Kinetic parameters of CmChi1

From: Molecular characterization of a novel chitinase CmChi1 from Chitinolyticbacter meiyuanensis SYBC-H1 and its use in N-acetyl-d-glucosamine production

Substrate

CC

p-NP-(GlcNAc)2

V max

526.2 ± 22.2 (μmol/min/L)

108.6 ± 3.3 (μmol/min/L)

K m

2.4 ± 0.12 (mg/mL)

0.58 ± 0.04 (μmol/mL)

k cat

18.6 ± 0.78 (s−1)

42.5 ± 1.4 (s−1)

kcat/Km

7.8 ± 0.11 (mL/s/mg)

73.5 ± 2.6 (mL/s/μmol)

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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