Fig. 3

Schematic illustration of the genome editing method for R. eutropha using electroporation-based CRISPR-Cas9 technique. The editing plasmid for genome was transferred into R. eutropha via electroporation, while 10 mg/mL fructose was added to the regeneration medium in the process of electroporation. Transformants were induced by 2 mg/mL l-arabinose for 96–168 h, then streaked on LB plate with 2 mg/mL l-arabinose and 200 µg/mL kanamycin. Before curing the plasmid, edited strains were identified by PCR and sequencing