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Table 4 Editing efficiencies of different loci on the R. eutropha genome

From: Genome editing of Ralstonia eutropha using an electroporation-based CRISPR-Cas9 technique

Genes

20 bp guide sequence

Deletion size (bp)

Edited colonies/total colonies

Editing efficiency (%)

rfp

catgcgtttcaaagttcgta

278

23/23

100

H16_A1814

tttcgcgaacctggcaaggt

599

18/23

78.3

H16_A1334

ctcggccgccttgctcatgt

330

23/23

100

H16_A1437

gcagggacatacggtgtttc

770

18/23

78.3

H16_B0204

cagatgccgccgtcgtacag

1031

22/23

95.7