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Table 2 Summary of the 16S rRNA gene sequencing data and α-diversity values in the fermentation cultures

From: Changes in the microbial consortium during dark hydrogen fermentation in a bioelectrochemical system increases methane production during a two-stage process

  BES MF (← BES) NBES MF (← NBES) MF
Cathode Anode
1 2 3 1 2 3 1 2 3 1 2 3 1 2 3 4
Read count 303,658 254,520 195,455 360,162 429,089 139,885 369,111 379,933 108,290 336,686 405,213 135,923 300,358 273,447 131,346 330,753
Observed OTUs 721 549 552 760 592 456 1767 1590 1012 757 396 328 1325 1326 900 1039
Chao1 1005 829 831 1019 837 726 2347 2141 1625 994 531 522 1829 1920 1462 1382
Shannon index 3.41 2.67 3.11 3.42 2.54 3.07 5.52 5.36 5.15 3.93 1.88 2.66 5.68 5.58 5.54 4.52
Simpson index 0.83 0.71 0.80 0.84 0.69 0.80 0.96 0.95 0.91 0.88 0.54 0.76 0.96 0.96 0.96 0.88
  1. The fermentation cultures were obtained from the BES (cathodic and anodic sides), NBES, subsequent MFs (← BES and ← NBES), and single MF. The α-diversity values included Chao1, Shannon index, and Simpson index. The numbers 1, 2, and 3, indicate the hydraulic retention time (HRT) and pH of the substrate (pHin) in the first stage for the two-stage process as follows; 1: 4 days and 6.5, 2: 2 days and 6.5, and 3: 2 days and 7.3. The number 4 indicates the HRT (4 days) and pHin (7.7) in the single-stage MF