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Table 1 Yeast strains used in this study

From: A pyruvate carbon flux tugging strategy for increasing 2,3-butanediol production and reducing ethanol subgeneration in the yeast Saccharomyces cerevisiae

Strain

Genotypes

Source

YPH499

MATa ura3-52 lys2-801 ade2-101 trp1-Δ63 his3-Δ200 leu2-Δ1

[27]

YJI2

YPH499 pdc5Δ

This study

YJI6

YPH499 pdc5Δ pdc6Δ

This study

YSM009

YPH499 pdc5Δ pdc6Δ MTH1-ΔT (genome sequence was not determined)

This study

YSM021 (PDCΔ)

YPH499 pdc1Δ pdc5Δ pdc6Δ MTH1-ΔT(L165F)

This study

Evo44

Obtained from YSM021 by laboratory evolution in SD medium (culture series 1) for 44 days

This study

Evo44-1

Colony #1 isolated from Evo44 glycerol stock

This study

Evo44-2

Colony #2 isolated from Evo44 glycerol stock

This study

Evo44-3

Colony #3 isolated from Evo44 glycerol stock

This study

Evo122

Obtained from YSM021 by laboratory evolution in SD medium (culture series 1) for 122 days

This study

Evo122-1

Colony #1 isolated from Evo122 glycerol stock

This study

Evo122-2

Colony #2 isolated from Evo122 glycerol stock

This study

Evo122-3

Colony #3 isolated from Evo122 glycerol stock

This study

YSM046 (PDCΔ + evolved)

Identical to Evo122-2: a laboratory-evolved yeast strain derived from PDCΔ (YSM021) strain

This study