Fig. 4

Assessment of medium-chain specific TE for diverting MCFA flux into TAG backbones. a MCFAs from the TAG fraction extracted from strain 2119 carrying different TEs. b TAG content and titer of strain 2119 carrying different TEs. c Fatty acid profile of the TAG fraction extracted from engineered strain 2119 carrying different TEs. d Time course of TAG production yielded by strain 2119 carrying pACYCDuet::RcFatB. Thin-layer chromatography (TLC) of lipid extracted from dried cells. e TAG profile and the distribution of C14 in the total TAGs from lipids extracted from strain 2119 and strain 2119 plus pACYCDuet::RcFatB, respectively. Strain 2119: E. coli BL21(DE3) carrying plasmid pCDFDuet::tDGAT/RoPAP::RoFadD1/RoTetR2. 48:1 represents 48 carbons with 1 double bond in three acyl chains. All data are the means ± standard deviations from triplicates