Fig. 5

TAG induction through nutrient level optimization. Contour profiles of TAG content (a) and TAG titer (b) for strain 2119 harboring pACYCDuet::RcFatB cultured in 16 designed media with different ratios of glycerol and N-Z-amine. Floating cells were observed during the collection of cells (c). Increases in the TAG/MCFA content (d, e) and titer (f) realized from nutrient optimization. The detailed information on designed media is shown in Additional file 10: Table S1. Cells were cultured at 37 °C with shaking at 200 rpm for 48 h. All data are the means ± standard deviations from triplicates