Fig. 1
From: An iterative computational design approach to increase the thermal endurance of a mesophilic enzyme
The addition of PDC to fermentative pathways in cellulolytic microbes can add a metabolic shortcut. The pathway shown highlights key steps in C. thermocellum fermentation metabolism. The conversion of glucose to pyruvate is summarized as Glycolysis. Enzymes are shown in boxes, redox cofactors in orange, and chemical species are underlined. PDC converts pyruvate directly to acetaldehyde, bypassing acetyl-CoA. Enzyme names are as follows: PFOR (pyruvate:ferredoxin oxidoreductase), PFL (pyruvate formate lyase), AldH (aldehyde dehydrogenase), AdhE (bifunctional aldehyde and alcohol dehydrogenase), and ADH (alcohol dehydrogenase)