Fig. 1From: Efficient heterologous expression of an alkaline lipase and its application in hydrolytic production of free astaxanthinExtracellular and intracellular proteins of four recombinant strains detected by Western blotting. Lanes 1–4: fermentation supernatants of zα-proalip-X33-1, mα-proalip-X33-1, mα-malip-X33-1, and zα-malip-X33-1. Lanes 5–8: broken-cell intracellular fluids of the four strains. Lane M: molecular weight markers (100, 60, 45, 28, 18 kDa). Arrow: target protein. Extracellular target proteins proALIP (30 kDa) and mALIP (26 kDa) (arrow) were found in broths of the four strains. Target proteins did not accumulate within cellsBack to article page