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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: Efficient heterologous expression of an alkaline lipase and its application in hydrolytic production of free astaxanthin

Fig. 1

Extracellular and intracellular proteins of four recombinant strains detected by Western blotting. Lanes 1–4: fermentation supernatants of zα-proalip-X33-1, mα-proalip-X33-1, mα-malip-X33-1, and zα-malip-X33-1. Lanes 5–8: broken-cell intracellular fluids of the four strains. Lane M: molecular weight markers (100, 60, 45, 28, 18 kDa). Arrow: target protein. Extracellular target proteins proALIP (30 kDa) and mALIP (26 kDa) (arrow) were found in broths of the four strains. Target proteins did not accumulate within cells

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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