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Table 4 Plasmids and strains used in this study

From: Efficient heterologous expression of an alkaline lipase and its application in hydrolytic production of free astaxanthin

Plasmid or strain Description Source
Plasmids
 pPICZαA Secretion expression vector with α-factor from S. cerevisiae [24]
 pPICMαA pPICZαA with α-factor optimized codons [24]
 pPICZαA-malip pPICZαA ligated with malip gene This study
 pPICZαA-proalip pPICZαA ligated with proalip gene This study
 pPICMαA-malip pPICMαA ligated with malip gene This study
 pPICMαA-proalip pPICMαA ligated with proalip gene This study
Strains
 X-33 Host strain (WT Mut+) [24]
 zα-malip-X33 malip of lipase without propeptide expressed in X-33 using pPICZαA This study
 zα-proalip-X33 proalip of lipase with propeptide expressed in X-33 using pPICZαA This study
 mα-malip-X33 malip expressed in X-33 using pPICMαA This study
 mα-proalip-X33 proalip expressed in X-33 using pPICMαA This study