Table 4 Plasmids and strains used in this study
Plasmid or strain | Description | Source |
|---|---|---|
Plasmids | ||
pPICZαA | Secretion expression vector with α-factor from S. cerevisiae | [24] |
pPICMαA | pPICZαA with α-factor optimized codons | [24] |
pPICZαA-malip | pPICZαA ligated with malip gene | This study |
pPICZαA-proalip | pPICZαA ligated with proalip gene | This study |
pPICMαA-malip | pPICMαA ligated with malip gene | This study |
pPICMαA-proalip | pPICMαA ligated with proalip gene | This study |
Strains | ||
X-33 | Host strain (WT Mut+) | [24] |
zα-malip-X33 | malip of lipase without propeptide expressed in X-33 using pPICZαA | This study |
zα-proalip-X33 | proalip of lipase with propeptide expressed in X-33 using pPICZαA | This study |
mα-malip-X33 | malip expressed in X-33 using pPICMαA | This study |
mα-proalip-X33 | proalip expressed in X-33 using pPICMαA | This study |