Type of model or algorithm | Target product | Model-based target: knockout gene A or overexpress gene B | References |
---|---|---|---|
Dynamic flux balance analysis | Ethanol | Ethanol production pathway after 20Â h | [163] |
Genome-scale metabolic model | Prediction of growth rate with respect to photosynthetic quotient for ethanol and butanol production | Double reaction knock out of hydrolyase and dehydrogenase | [164] |
In vivo carbon flux analysis | Homolanthionine | Deletion of methionine and cysteine biosynthesis repressor protein | [165] |
Metabolic flux analysis | Hydrogen production | Deletion and addition of GAP1 and dehydrogenase, respectively | [166] |
Metabolic flux analysis | Astaxanthin synthesis | – | [167] |
Metabolic flux analysis | Fixed more CO2 and had a higher biomass yield | Alternate pathway for isoleucine synthesis (via citramalate synthase, CimA | [168] |
Metabolic net fluxes | Hydrogen production | Disruption of hydrogenase and poly-β-hydroxybutyrate synthase | [169] |