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Table 1 Properties of the studied endomannanases

From: Boosting of enzymatic softwood saccharification by fungal GH5 and GH26 endomannanases

Origin Domains Mwa pH bopt Relative activity Tmc t½d Sequence IDe Identityf
kDa pH5/pHopt  °C h %
GH26
 Collariella virescens (CvirMan26A) CBM35-GH26-CBM1 57.9 6 (5–7) 0.97 62 BBW45415 76
 Mycothermus thermophiles (MtheMan26A) CBM35-GH26 52.1 5 (5–8) 1.00 68 91 ± 0.3 MH208368 76
 Podospora anserina (PansMan26A) CBM35-GH26 49.8 6 (5–7) 0.97 57 90 ± 5.5 B2AEP0, [24] 100
 Podospora anserina (PansMan26A core) GH26 34.4 5 (5–7) 1.00 58 103 ± 2.2 (B2AEP0) 100
 Neoascochyta desmazieri (NdesMan26A) CBM35-GH26 48.7 5 (4–7) 1.00 65 267 ± 24.1 MH208367 60
 Westerdykella sp. (Wsp.Man26A) CBM35-GH26 50.4 6 (6–7) 0.79 58 59 ± 4.3 MH208369 55
 Ascobolus stictoideus (AstiMan26A) CBM35-GH26-CBM1 59.4 7 (5–7) 0.80 61 81 ± 7.7 BBW45412 55
 Aspergillus nidulans (AnidMan26A) GH26 35.2 6 (5–7) 0.93 53 10 ± 0.1 Q5AWB7, [26] 52
 Yunnania penicillata (YpenMan26A) GH26 34.5 6 (5–8) 0.87 50 21 ± 0.1 BDN98740 48
GH5
 Trichoderma reesei (TresMan5A) GH5-CBM1 45.2 4 (4–5) 0.93 81 Q99036, [27] 36
 Trichoderma reesei (TresMan5A core) GH5 38.8 4 (3–6) 0.88 78 2390 ± 360 (Q99036), [27] 36
 Aspergillus niger (AnigMan5A) GH5 39.8 4 (3–5) 0.85 87 137 ± 10.0 BCK48306, [36] 30
  1. aTheoretical (non-glycosylated protein)
  2. bpH optimum at 37 °C and pH interval with 80% relative activity in brackets
  3. cThe thermal midpoint (Tm) at pH 5
  4. dHalf-lives (t½) at 30 °C. No decay was observed for CvirMan26A and TresMan5A during the 48 h incubation period (Additional file 1: Figure S1)
  5. eA reference is given when the enzyme is previously characterized
  6. fHomology to PansMan26A for which the structure is known (PDB ID: 3ZM8)