From: Boosting of enzymatic softwood saccharification by fungal GH5 and GH26 endomannanases
Origin | Domains | Mwa | pH bopt | Relative activity | Tmc | t½d | Sequence IDe | Identityf |
---|---|---|---|---|---|---|---|---|
kDa | pH5/pHopt | °C | h | % | ||||
GH26 | ||||||||
Collariella virescens (CvirMan26A) | CBM35-GH26-CBM1 | 57.9 | 6 (5–7) | 0.97 | 62 | – | BBW45415 | 76 |
Mycothermus thermophiles (MtheMan26A) | CBM35-GH26 | 52.1 | 5 (5–8) | 1.00 | 68 | 91 ± 0.3 | MH208368 | 76 |
Podospora anserina (PansMan26A) | CBM35-GH26 | 49.8 | 6 (5–7) | 0.97 | 57 | 90 ± 5.5 | B2AEP0, [24] | 100 |
Podospora anserina (PansMan26A core) | GH26 | 34.4 | 5 (5–7) | 1.00 | 58 | 103 ± 2.2 | (B2AEP0) | 100 |
Neoascochyta desmazieri (NdesMan26A) | CBM35-GH26 | 48.7 | 5 (4–7) | 1.00 | 65 | 267 ± 24.1 | MH208367 | 60 |
Westerdykella sp. (Wsp.Man26A) | CBM35-GH26 | 50.4 | 6 (6–7) | 0.79 | 58 | 59 ± 4.3 | MH208369 | 55 |
Ascobolus stictoideus (AstiMan26A) | CBM35-GH26-CBM1 | 59.4 | 7 (5–7) | 0.80 | 61 | 81 ± 7.7 | BBW45412 | 55 |
Aspergillus nidulans (AnidMan26A) | GH26 | 35.2 | 6 (5–7) | 0.93 | 53 | 10 ± 0.1 | Q5AWB7, [26] | 52 |
Yunnania penicillata (YpenMan26A) | GH26 | 34.5 | 6 (5–8) | 0.87 | 50 | 21 ± 0.1 | BDN98740 | 48 |
GH5 | ||||||||
Trichoderma reesei (TresMan5A) | GH5-CBM1 | 45.2 | 4 (4–5) | 0.93 | 81 | – | Q99036, [27] | 36 |
Trichoderma reesei (TresMan5A core) | GH5 | 38.8 | 4 (3–6) | 0.88 | 78 | 2390 ± 360 | (Q99036), [27] | 36 |
Aspergillus niger (AnigMan5A) | GH5 | 39.8 | 4 (3–5) | 0.85 | 87 | 137 ± 10.0 | BCK48306, [36] | 30 |