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Table 1 Properties of the studied endomannanases

From: Boosting of enzymatic softwood saccharification by fungal GH5 and GH26 endomannanases

Origin

Domains

Mwa

pH bopt

Relative activity

Tmc

t½d

Sequence IDe

Identityf

kDa

pH5/pHopt

 °C

h

%

GH26

 Collariella virescens (CvirMan26A)

CBM35-GH26-CBM1

57.9

6 (5–7)

0.97

62

BBW45415

76

 Mycothermus thermophiles (MtheMan26A)

CBM35-GH26

52.1

5 (5–8)

1.00

68

91 ± 0.3

MH208368

76

 Podospora anserina (PansMan26A)

CBM35-GH26

49.8

6 (5–7)

0.97

57

90 ± 5.5

B2AEP0, [24]

100

 Podospora anserina (PansMan26A core)

GH26

34.4

5 (5–7)

1.00

58

103 ± 2.2

(B2AEP0)

100

 Neoascochyta desmazieri (NdesMan26A)

CBM35-GH26

48.7

5 (4–7)

1.00

65

267 ± 24.1

MH208367

60

 Westerdykella sp. (Wsp.Man26A)

CBM35-GH26

50.4

6 (6–7)

0.79

58

59 ± 4.3

MH208369

55

 Ascobolus stictoideus (AstiMan26A)

CBM35-GH26-CBM1

59.4

7 (5–7)

0.80

61

81 ± 7.7

BBW45412

55

 Aspergillus nidulans (AnidMan26A)

GH26

35.2

6 (5–7)

0.93

53

10 ± 0.1

Q5AWB7, [26]

52

 Yunnania penicillata (YpenMan26A)

GH26

34.5

6 (5–8)

0.87

50

21 ± 0.1

BDN98740

48

GH5

 Trichoderma reesei (TresMan5A)

GH5-CBM1

45.2

4 (4–5)

0.93

81

Q99036, [27]

36

 Trichoderma reesei (TresMan5A core)

GH5

38.8

4 (3–6)

0.88

78

2390 ± 360

(Q99036), [27]

36

 Aspergillus niger (AnigMan5A)

GH5

39.8

4 (3–5)

0.85

87

137 ± 10.0

BCK48306, [36]

30

  1. aTheoretical (non-glycosylated protein)
  2. bpH optimum at 37 °C and pH interval with 80% relative activity in brackets
  3. cThe thermal midpoint (Tm) at pH 5
  4. dHalf-lives (t½) at 30 °C. No decay was observed for CvirMan26A and TresMan5A during the 48 h incubation period (Additional file 1: Figure S1)
  5. eA reference is given when the enzyme is previously characterized
  6. fHomology to PansMan26A for which the structure is known (PDB ID: 3ZM8)