Fig. 7

One-pot batch cultivation of ADP1-g and C. butyricum in 1-l bioreactor. The cocultivation was carried out in 800 ml of initially aerobic JM medium supplemented with 20 mM glucose. The changes in (a) pH (closed square), pO₂ (asterisk) and biomass formation (in cell dry weight, CDW; open circle), and (b) glucose (open square), acetate (open star), butyrate (open triangle) and wax ester concentrations (in bars) are presented. C. butyricum consumed glucose producing acetate and butyrate during the first 24 h (anaerobic phase), whereas ADP1-g utilized the fermentation end-products during the following 10 h (aerobic phase). Arrows indicate the timepoint at which the oxygen supply was initiated and the culture pH adjusted to 7.3. The data for CDW, glucose, acetate and butyrate are mean values from triplicate technical repeats. The standard deviations (error bars) of each data are plotted and in some cases the symbols overlap the error bars