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TableĀ 1 Strains and plasmids used in this study

From: Iterative cycle of widely targeted metabolic profiling for the improvement of 1-butanol titer and productivity in Synechococcus elongatus

Ā 

Genotype characteristic

References

Cyanobacteria strain

Ā PCC 7942

Wild-type Synechococcus elongatus PCC 7942

ā€“

Ā EL22

PTrc:: His-tagged T. denticola ter integrated at NSI and PLlacO1:: nphT7, bldh, yqhD, phaJ, phaB integrated at NSII in PCC 7942 genome

[15]

Ā BUOHSE

PTrc:: His-tagged T. denticola ter integrated at NSI and PLlacO1:: nphT7, pduP_S. enterica, yqhD, phaJ, phaB integrated at NSII

[16]

Ā BUOHSE without pduP

PTrc:: His-tagged T. denticola ter integrated at NSI and PLlacO1:: nphT7, phaJ, phaB integrated at NSII

[18]

Ā DC7

PTrc:: His-tagged T. denticola ter integrated at NSI and PLlacO1:: nphT7, PduP_S. enterica, yqhD, phaJ, phaB integrated at NSII, synthetic RBS TIR: 522,266.72 in front of pduP

This work

Ā DC11

PTrc:: His-tagged T. denticola ter integrated at NSI and PLlacO1:: nphT7, pduP_S. enterica, yqhD, phaJ, phaB integrated at NSII, synthetic RBS TIR: 522,266.72 in front of pduP

PLlacO1: ACCase integrated at aldA

This work

Plasmid

Ā pSR3

KanR; NSII targeting; PLlacO1:: nphT7, yqhD, pduP, phaJ, phaB

[16]

Ā pEL256

KanR; NSII targeting; PLlacO1:: nphT7, phaJ, phaB

[18]

Ā pDC304

KanR; NSII targeting; PLlacO1:: nphT7, yqhD, synthetic RBS for pduP, phaJ, phaB

This work

Ā pDC331

GenR; aldA targeting; PLlacO1: ACCase Yarrowia lipolytica integrated at aldA

This work

  1. nphT7 (Streptomyces sp. Strain CL190), acetoacetyl-CoA synthase; bldh (C. saccharoperbutylacetonicum), CoA-acylating butanal dehydrogenase; yqhD (E. coli), NADPH-dependent alcohol dehydrogenase; phaJ (A. caviae), (R)-specific crotonase; phaB (R. eutropha), acetoacetyl-CoA reductase; pduP (S. enterica), CoA-acylating propionaldehyde dehydrogenase; ACCase (Yarrowia lipolytica), acetyl-CoA carboxylase. KanR kanamycin resistance, GenR gentamycin resistance