Ā | Genotype characteristic | References |
---|---|---|
Cyanobacteria strain | ||
Ā PCC 7942 | Wild-type Synechococcus elongatus PCC 7942 | ā |
Ā EL22 | PTrc:: His-tagged T. denticola ter integrated at NSI and PLlacO1:: nphT7, bldh, yqhD, phaJ, phaB integrated at NSII in PCC 7942 genome | [15] |
Ā BUOHSE | PTrc:: His-tagged T. denticola ter integrated at NSI and PLlacO1:: nphT7, pduP_S. enterica, yqhD, phaJ, phaB integrated at NSII | [16] |
Ā BUOHSE without pduP | PTrc:: His-tagged T. denticola ter integrated at NSI and PLlacO1:: nphT7, phaJ, phaB integrated at NSII | [18] |
Ā DC7 | PTrc:: His-tagged T. denticola ter integrated at NSI and PLlacO1:: nphT7, PduP_S. enterica, yqhD, phaJ, phaB integrated at NSII, synthetic RBS TIR: 522,266.72 in front of pduP | This work |
Ā DC11 | PTrc:: His-tagged T. denticola ter integrated at NSI and PLlacO1:: nphT7, pduP_S. enterica, yqhD, phaJ, phaB integrated at NSII, synthetic RBS TIR: 522,266.72 in front of pduP PLlacO1: ACCase integrated at aldA | This work |
Plasmid | ||
Ā pSR3 | KanR; NSII targeting; PLlacO1:: nphT7, yqhD, pduP, phaJ, phaB | [16] |
Ā pEL256 | KanR; NSII targeting; PLlacO1:: nphT7, phaJ, phaB | [18] |
Ā pDC304 | KanR; NSII targeting; PLlacO1:: nphT7, yqhD, synthetic RBS for pduP, phaJ, phaB | This work |
Ā pDC331 | GenR; aldA targeting; PLlacO1: ACCase Yarrowia lipolytica integrated at aldA | This work |