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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Metabolic engineering of Saccharomyces cerevisiae for 7-dehydrocholesterol overproduction

Fig. 3

Fine-tuning the transcription level of DHCR24 to enhance 7-DHC production. a Real-Time PCR analysis of the transcription levels of DHCR24 in strains harvested at 10 h (orange, glucose consumption phase) and 30 h (blue, ethanol consumption phase). The relative transcription level for each gene was determined as 2−ΔΔCt using gene ALG9 for normalization. All data were from at least triplicate experiments. b Effects of the induction strategy, genomic integration site, and promoter of DHCR24 on 7-DHC accumulation

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