Table 1 S. cerevisiae strains used in this study
From: Metabolic engineering of Saccharomyces cerevisiae for 7-dehydrocholesterol overproduction
Strain | Description | Source |
|---|---|---|
CEN.PK2-1D | MATα, URA3-52, TRP1-289, LEU2-3112, HIS3Δ1, MAL2-8C, SUC2 | EUROSCARF |
SyBE_Sc01130007 | CEN.PK2-1D, LEU2::BieR-ERG19-PGAL1,10-ERG8, ADE1::tHMG1-PGAL1,10-IDI1_ADE1, HIS3::HIS3-ERG12-PGAL1,10-ERG10, URA3::tHMG1- PGAL1,10-ERG13-URA3, TRP1::tHMG1- PGAL1,10-ERG20-TPR1, GAL1,7,10::HphR | This study |
SyBE_Sc0125XJ01 | SyBE_Sc01130007, ∆ERG5 | This study |
SyBE_Sc01250050 | SyBE_Sc0125XJ01, GAL80::PGAL7-Hs_DHCR24-TPGK1-LEU2 | This study |
SyBE_Sc01250001 | SyBE_Sc0125XJ01, GAL80::PGAL7- Cg_DHCR24-TPGK1-LEU2 | This study |
SyBE_Sc01250002 | SyBE_Sc0125XJ01, GAL80::PGAL7- Tg_DHCR24-TPGK1-LEU2 | This study |
SyBE_Sc01250003 | SyBE_Sc0125XJ01, GAL80::PGAL7-Mm_DHCR24-TPGK1-LEU2 | This study |
SyBE_Sc01250004 | SyBE_Sc0125XJ01, GAL80::PGAL7- At_DHCR24-TPGK1-LEU2 | This study |
SyBE_Sc01250006 | SyBE_Sc0125XJ01, GAL80::PGAL7-Dr_DHCR24-TPGK1-LEU2 | This study |
SyBE_Sc01250007 | SyBE_Sc0125XJ01, GAL80::PGAL7- Gh_DHCR24-TPGK1-LEU2 | This study |
SyBE_Sc01250008 | SyBE_Sc0125XJ01, GAL80::PGAL7-Ec_DHCR24-TPGK1-LEU2 | This study |
SyBE_Sc01250009 | SyBE_Sc0125XJ01, GAL80::PGAL7-Gg_DHCR24-TPGK1-LEU2 | This study |
SyBE_Sc01250010 | SyBE_Sc0125XJ01, GAL80::PGAL7-Xt_DHCR24-TPGK1-LEU2 | This study |
SyBE_Sc01250011 | SyBE_Sc0125XJ01, GAL80::PGAL7-Bt_DHCR24-TPGK1-LEU2 | This study |
SyBE_Sc0125H001 | SyBE_Sc0125XJ01, GAL80::PGAL7-Cg DHCR24-6HIS-TPGK1-LEU2 | This study |
SyBE_Sc0125H002 | SyBE_Sc0125XJ01, GAL80::PGAL7-Tg DHCR24-6HIS-TPGK1-LEU2 | This study |
SyBE_Sc0125H003 | SyBE_Sc0125XJ01, GAL80::PGAL7-Mm DHCR24-6HIS-TPGK1-LEU2 | This study |
SyBE_Sc0125H005 | SyBE_Sc0125XJ01, GAL80::PGAL7-At DHCR24-6HIS-TPGK1-LEU2 | This study |
SyBE_Sc0125H007 | SyBE_Sc0125XJ01, GAL80::PGAL7-Dr DHCR24-6HIS-TPGK1-LEU2 | This study |
SyBE_Sc0125H009 | SyBE_Sc0125XJ01, GAL80::PGAL7-Gh DHCR24-6HIS-TPGK1-LEU2 | This study |
SyBE_Sc0125H010 | SyBE_Sc0125XJ01, GAL80::PGAL7-Ec DHCR24-6HIS-TPGK1-LEU2 | This study |
SyBE_Sc0125H011 | SyBE_Sc0125XJ01, GAL80::PGAL7-Gg DHCR24-6HIS-TPGK1-LEU2 | This study |
SyBE_Sc0125H012 | SyBE_Sc0125XJ01, GAL80::PGAL7-Xt DHCR24-6HIS-TPGK1-LEU2 | This study |
SyBE_Sc0125H013 | SyBE_Sc0125XJ01, GAL80::PGAL7-Bt DHCR24-6HIS-TPGK1-LEU2 | This study |
SyBE_Sc0125H050 | SyBE_Sc0125XJ01, GAL80::PGAL7-Hs DHCR24-6HIS-TPGK1-LEU2 | This study |
SyBE_Sc0125XJ02 | SyBE_Sc0125XJ01, GAL7,10,1::PGAL7-Gg_DHCR24-TCYC1-URA3 | This study |
SyBE_Sc0125XJ03 | SyBE_Sc0125XJ01, GAL7,10,1::PGAL1-Gg_DHCR24-TCYC1-URA3 | This study |
SyBE_Sc0125X001 | SyBE_Sc0125XJ01, GAL7,10,1::PGAL1-Gg_DHCR24-TCYC1 | This study |
SyBE_Sc0125XJ04 | SyBE_Sc0125XJ01, GAL7,10,1::PGAL1-Gg_DHCR24-TCYC1, ERG6::LEU2 | This study |
SyBE_Sc0125XJ06 | SyBE_Sc0125XJ01, GAL7,10,1::PGAL1-Gg_DHCR24-TCYC1; ∆ERG6::PGAL1-Gg_DHCR24-TCYC1-LEU2 | This study |
SyBE_Sc0125XJ07 | SyBE_Sc0125XJ06, FLD1::URA3 | This study |
SyBE_Sc0125XJ08 | SyBE_Sc0125XJ06, NEM1::URA3 | This study |
SyBE_Sc0125XJ09 | SyBE_Sc0125XJ06, FLD1::KanMX, NEM1::URA3 | This study |