Fig. 2
Effect of various feeding regimes for H2O2 and reductant (AscA) on Avicel oxidation by LPMOs present in Cellic® CTec2. The various feeding scenarios are shown in the Table below the Figure. Note the positive control reaction (7, green: aerobic, 1 mM AscA, no H2O2 added) and the two negative control reactions (6, gray: anaerobic, 1 mM AscA, no H2O2 added; 5, purple: anaerobic, no AscA, with repetitive addition of 200 µM H2O2). AscA and/or H2O2 were added after 60, 120, 180 and/or 240 min as indicated in the Table. The blue dashed line represents the concentration of AscA (secondary y-axis) measured in reaction 1 (blue line; anaerobic, 1 mM AscA with repetitive addition of 200 µM H2O2). The reactions mixtures contained 10% (w/w) DM of Avicel and 4 mg/g DM of Cellic® CTec2, in 50 mM sodium acetate buffer at pH 5.0 and were incubated at 50 °C. The error bars represent standard deviations for three independent experiments