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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: The impact of hydrogen peroxide supply on LPMO activity and overall saccharification efficiency of a commercial cellulase cocktail

Fig. 3

Effect of the H2O2 feeding rate on the initial phase of saccharification of Avicel with Cellic® CTec2. a, d The production of Glc4gemGlc, b, e The extent of glucan conversion and c, f the concentration of AscA. Unless otherwise stated, reactions were carried out with 10% (w/w) DM of Avicel, 4 mg/g DM of Cellic® CTec2 and 1 mM of AscA, in 50 mM sodium acetate buffer at pH 5.0, at 50 °C. The reactions displayed in df were conducted with the following modifications (marked a, b or c): aindicates the absence of AscA, bindicates the presence of 0.1 mM AscA, and cindicates the use of 2 mg/g DM of Cellic® CTec2. H2O2 was supplied at a constant flow rate of 600 µL h−1 using H2O2 stock solutions with appropriate concentrations (see Table 1 for details) to obtain the desired H2O2 feeding rates (µM h−1). To ensure anaerobic conditions, all reactions were performed with constant sparging of nitrogen at a flow rate of 100 mL min−1, with exception of the aerobic control reaction (labeled “O 2 ), which was sparged with air at the same flow rate. “N2” stands for the anaerobic control reaction. Both control reactions were run without the addition of H2O2. Repeated addition of AscA to a final concentration of 1 mM, in the reaction fed with 3000 µM h−1, is marked with a blue asterisk (*). Error bars for glucan conversion represent standard deviations of two technical replicates. The data depicted in ac, except the data for the highest H2O2 feeding rate, have been published previously [29]

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