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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: The impact of hydrogen peroxide supply on LPMO activity and overall saccharification efficiency of a commercial cellulase cocktail

Fig. 5

Effect of various constant H2O2 feeding rates on the saccharification of sulfite-pulped Norway spruce with Cellic® CTec2. a The production of Glc4gemGlc and b the glucan conversion. All reactions were carried out with 10% (w/w) DM of spruce, 4 mg/g DM of Cellic® CTec2 and 1 mM of AscA, in 50 mM sodium acetate buffer at pH 5.0 and 50 °C with constant supply of H2O2 (600 µL h−1) using H2O2 stock solutions with appropriate concentrations (Table 1). To ensure anaerobic conditions, all reactions were carried out with constant sparging of nitrogen at a flow rate of 100 mL min−1, with the exception of the aerobic control reaction (O2), which was sparged with air at the same flow rate. “N2” stands for the anaerobic control reaction. Both control reactions were run without addition of H2O2. Error bars for glucan conversion represent standard deviations of two technical replicates

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