Table 3 Levels of the factors evaluated in the \(2_{\text{V}}^{{ 5 { - 1}}}\) fractional factorial design, sample identification with the corresponding experimental conditions and the main response evaluated in the organosolv pretreatment applied to elephant grass leaves (EO)
From: Optimization of biomass pretreatments using fractional factorial experimental design
Low level (−) | High level (+) | Central (0) | |
|---|---|---|---|
Factor levels | |||
A-[H2SO4] (%v/v) | None | 1 | 0.5 |
B-[catalyst] (mol/L) | 0 | 0.06 | 0.03 |
C-[ethanol] (%v/v) | 40 | 80 | 60 |
D-temperature (°C) | 160 | 200 | 180 |
E-time (min) | 30 | 90 | 60 |
Sample name | Samples and experimental conditions | Responses | ||||
|---|---|---|---|---|---|---|
[H2SO4] step 1 (% v/v) | [Catalyst] step 2 (mol/L) | [Ethanol] step 2 (% v/v) | Temp step 2 (°C) | Time step 2 (min) | Reducing sugar release (mg/g) | |
EO1 | None | None | 40 | 160 | 90 | 58.5 |
EO2 | 1 | None | 40 | 160 | 30 | 95.1 |
EO3 | None | 0.06 | 40 | 160 | 30 | 98.0 |
EO4 | 1 | 0.06 | 40 | 160 | 90 | 102.9 |
EO5 | None | None | 80 | 160 | 30 | 43.6 |
EO6 | 1 | None | 80 | 160 | 90 | 91.7 |
EO7 | None | 0.06 | 80 | 160 | 90 | 143.6 |
EO8 | 1 | 0.06 | 80 | 160 | 30 | 120.6 |
EO9 | None | None | 40 | 200 | 30 | 77.3 |
EO10 | 1 | None | 40 | 200 | 90 | 82.2 |
EO11 | None | 0.06 | 40 | 200 | 90 | 59.2 |
EO12 | 1 | 0.06 | 40 | 200 | 30 | 56.1 |
EO13 | None | None | 80 | 200 | 90 | 90.3 |
EO14 | 1 | None | 80 | 200 | 30 | 73.7 |
EO15 | None | 0.06 | 80 | 200 | 30 | 109.9 |
EO16 | 1 | 0.06 | 80 | 200 | 90 | 5.0 |
EO17 | 0.5 | 0.03 | 60 | 180 | 60 | 100.4 |
EO18 | 0.5 | 0.03 | 60 | 180 | 60 | 106.1 |
EO19 | 0.5 | 0.03 | 60 | 180 | 60 | 95.6 |