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Table 2 Expression levels of the genes in strains with deletion or overexpression of DAS1 or TAL2 relative to the parental wild-type strain on the third day of xylose alcoholic fermentation at 45 °C

From: Peroxisomes and peroxisomal transketolase and transaldolase enzymes are essential for xylose alcoholic fermentation by the methylotrophic thermotolerant yeast, Ogataea (Hansenula) polymorpha

Strains

Genes

XYL1

XYL2

XYL3

PDC1

RPE1

TKL1

ADH1

CYC1

FBP1

PCK1

das1∆/WT

0.295 ± 0.072

1.330 ± 0.044

1.491 ± 0.111

1.253 ± 0.110

1.261 ± 0.267

1.384 ± 0.169

0.861 ± 0.032

1.397 ± 0.065

1.738 ± 0.056

1.462 ± 0.019

DAS1*/WT

2.155 ± 0.232

1.044 ± 0.134

0.801 ± 0.015

0.713 ± 0.006

0.877 ± 0.056

1.337 ± 0.041

0.571 ± 0.125

1.099 ± 0.088

0.309 ± 0.099

0.447 ± 0.098

tal2∆/WT

0.124 ± 0.062

1.538 ± 0.016

0.909 ± 0.181

0.717 ± 0.104

0.830 ± 0.072

0.867 ± 0.111

0.609 ± 0.128

1.576 ± 0.205

1.901 ± 0.049

1.44 ± 0.051

TAL2*/WT

1.452 ± 0.234

1.301 ± 0.246

1.158 ± 0.092

0.580 ± 0.101

0.575 ± 0.161

0.667 ± 0.122

1.246 ± 0.081

1.689 ± 0.133

1.428 ± 0.085

0.837 ± 0.114

  1. The mRNA quantification was normalized to ACT1 mRNA