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Table 2 Mutations of the ALE-9.0 compared to WT

From: Adaptive laboratory evolution of cadmium tolerance in Synechocystis sp. PCC 6803

Position

Gene

Mutation

Protein

Mutated in 7.0 µM

Nucleotide

Protein

3506559

slr0454

SV

Early termination

Cation or drug efflux system protein

N

2961208

slr0623

T–C

P30L

Thioredoxin

Y

99738

slr0721

G–A

M113V

Malic enzyme

N

2401781

slr0774

A–G

G270R

Protein-export membrane protein SecD

Y

3061976

slr0798

G–A

Y207C

Zinc-transporting P-type ATPase involved in zinc tolerance

Y

306570

slr1302

G–C

P299A

Protein involved in constitutive low affinity CO2 uptake

N

1135407

ssr1480

G–A

Y24C

Putative RNA-binding protein

Y

1500784

sll1586

C–T

L496L

Unknown protein

Y

527831

slr1753

Ins(GAACCC)

1163PE

Hypothetical protein

Y