Fig. 2From: Engineering TATA-binding protein Spt15 to improve ethanol tolerance and production in Kluyveromyces marxianusSpotting test of mutant strains M2 and M10 at 45 °C under different concentrations of ethanol. 2 μL cell suspensions of each strain with OD600 of 0.20 and serial dilutions of 10−1 to 10−3 were spotted onto YPD agar medium and then incubated at 45 °C. SPT15 stands for the strain overexpressing wild-type SPT15Back to article page