Use of agro-industrial residue from the canned pineapple industry for polyhydroxybutyrate production by Cupriavidus necator strain A-04

Table 2 Effect of particle sizes on the yield of sugars

Pineapple wastes	Particle size (mm)	Sugars (g/L)					Total FS (g/L)	\(Y_{{\text{FS/PW}}}\) (g/g)	S_PHB (g/L)	\(Y_{{S_{{\text{PHB}}} /\text{PW}}}\) (g/g)
Pineapple wastes	Particle size (mm)	Glucose	Fructose	Galactose	Arabinose	Xylose	Total FS (g/L)	\(Y_{{\text{FS/PW}}}\) (g/g)	S_PHB (g/L)	\(Y_{{S_{{\text{PHB}}} /\text{PW}}}\) (g/g)
1.5% (v/v) H₂SO₄
Core	> 0.841	24.5 ± 0.1	12.4 ± 0.6	8.1 ± 0.6	10.4 ± 0.2	10.3 ± 0.6	65.7 ± 0.4	0.66	36.9 ± 0.4	0.37
	0.841–0.420	31.0 ± 0.1	2.6 ± 0.9	7.6 ± 0.6	8.3 ± 0.6	31.5 ± 0.3	81.0 ± 0.5	0.81	33.6 ± 0.5	0.34
	< 0.420	22.6 ± 0.7	4.0 ± 0.3	8.4 ± 0.4	10.5 ± 0.5	9.1 ± 0.6	54.6 ± 0.5	0.55	26.6 ± 0.5	0.27
Peel	> 0.841	35.8 ± 0.4	7.1 ± 0.4	6.6 ± 0.2	5.3 ± 0.3	13.1 ± 0.9	67.9 ± 0.4	0.68	43.9 ± 0.4	0.44
	0.841–0.420	17.5 ± 0.4	1.5 ± 0.1	5.6 ± 0.1	4.6 ± 0.1	24.1 ± 0.4	53.3 ± 0.2	0.53	19.6 ± 0.3	0.20
	< 0.420	15.3 ± 0.4	1.6 ± 0.1	6.0 ± 0.1	6.6 ± 0.4	15.3 ± 0.4	44.8 ± 0.3	0.45	16.9 ± 0.3	0.17
1.5% (v/v) H₃PO₄
Core	> 0.841	31.4 ± 1.0	25.8 ± 1.1	4.8 ± 0.4	4.1 ± 0.3	3.6 ± 0.4	69.7 ± 0.6	0.70	57.2 ± 1.0	0.57
Peel	> 0.841	20.6 ± 0.8	8.0 ± 0.5	6.2 ± 0.9	8.2 ± 0.3	15.7 ± 0.7	58.7 ± 0.6	0.69	28.6 ± 0.6	0.29

Ten grams of samples for each of three particle sizes were hydrolyzed with 1.5% (v/v) H₂SO₄ and 1.5% (v/v) H₃PO₄ at 121 °C for 15 min
S_PHB PHB substrates, FS fermentable sugars, PW pineapple waste

ISSN: 2731-3654