TY - JOUR AU - Yoav, Shahar AU - Salame, Tomer M. AU - Feldman, Daria AU - Levinson, Dana AU - Ioelovich, Michael AU - Morag, Ely AU - Yarden, Oded AU - Bayer, Edward A. AU - Hadar, Yitzhak PY - 2018 DA - 2018/07/27 TI - Effects of cre1 modification in the white-rot fungus Pleurotus ostreatus PC9: altering substrate preference during biological pretreatment JO - Biotechnology for Biofuels SP - 212 VL - 11 IS - 1 AB - During the process of bioethanol production, cellulose is hydrolyzed into its monomeric soluble units. For efficient hydrolysis, a chemical and/or mechanical pretreatment step is required. Such pretreatment is designed to increase enzymatic digestibility of the cellulose chains inter alia by de-crystallization of the cellulose chains and by removing barriers, such as lignin from the plant cell wall. Biological pretreatment, in which lignin is decomposed or modified by white-rot fungi, has also been considered. One disadvantage in biological pretreatment, however, is the consumption of the cellulose by the fungus. Thus, fungal species that attack lignin with only minimal cellulose loss are advantageous. The secretomes of white-rot fungi contain carbohydrate-active enzymes (CAZymes) including lignin-modifying enzymes. Thus, modification of secretome composition can alter the ratio of lignin/cellulose degradation. SN - 1754-6834 UR - https://doi.org/10.1186/s13068-018-1209-6 DO - 10.1186/s13068-018-1209-6 ID - Yoav2018 ER -