Fig. 3
Production of C5, C10, and C15 isoprenoid alcohols and their derivatives in engineered E. coli. a Production and fractional compositions (%) of IPP/DMAPP (C5)- and FPP (C15)-derived isoprenoids from strains NA-MBF1.0 and NAK-MBF1.0. Strains NA-MBF1.0 and NAK-MBF1.0 are E. coli DH5α strains harboring pSNA and pSNAK, respectively, along with pT-MBF1.0. b Production and fractional compositions (%) of IPP/DMAPP (C5)-, GPP (C10)-, and FPP (C15)-derived isoprenoids from strains NA-MBF2.0 and NAK-MBF2.0. Strains NA-MBF2.0 and NAK-MBF2.0 were transformed with pSNA and pSNAK, respectively, along with pT-MBF2.0. Cultures were incubated at 30 °C for 48 h in 2YT medium containing 2.0% (v/v) glycerol and were initially induced with 0.5 mM IPTG. The error bars represent the range from three independent experiments