Fig. 4
An optimized synthetic pathway for the production of C5 and C15 isoprenoid alcohols and their derivatives. Production of IPP/DMAPP (C5)- and FPP (C15)-derived isoprenoids from strains NA-MBF1.1, NA-MBF1.2, NA-MBF1.1a, and NA-MBF1.2a harboring pT-MBF1.1, pT-MBF1.2, pT-MBF1.1a, and pT-MBF1.2a, respectively, in addition to pSNA. Cultures were incubated at 30 °C for 48 h in 2YT medium containing 2.0% (v/v) glycerol and were initially induced with 0.5 mM IPTG. The error bars represent the range from three independent experiments. The two dotted lines represent the production of C5 products (black) and C15 products (orange) from strain NA-MBF1.0