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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: The mechanism by which a distinguishing arabinofuranosidase can cope with internal di-substitutions in arabinoxylans

Fig. 4

XacAbf51 hydrolyzes internal mono- and di-substitutions of AXOS. a Mass spectra of XA2+3XX (23, 33-di-α-l-arabinofuranosyl-xylotetraose) after 0, 10 and 15 min of reaction with XacAbf51 (15 µg mL−1) at 50 °C, pH 5.5, using 10 mM substrate. Boxed peaks correspond to the sodiated forms of the substrate XA2+3XX (m z−1 = 810 + 23 (Na+) = 833) and the product xylotetraose (XXXX, m/z = 546 + 23 (Na+) = 569). b Kinetic parameters of XA2+3XX hydrolysis by XacAbf51 (15 µg mL−1) at 50 °C, pH 5.5, assessed by mass spectrometry in triplicate. c Mass spectra of XA3XX (33-α-l-arabinofuranosyl-xylotetraose) after 0, 10 and 15 min of reaction with XacAbf51 in the same conditions described in (a). Boxed peaks correspond to the sodiated forms of the substrate XA3XX (m z−1 = 678 + 23 (Na+) = 701) and the product XXXX (m/z = 546 + 23 (Na+) = 569). d Kinetic parameters of XA3XX hydrolysis by XacAbf51 (15 µg mL−1) at 50 °C, pH 5.5, assessed by mass spectrometry in triplicate. Error bar represents standard deviations of the mean

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