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Table 1 Kinetic parameters of XacAbf51 and TxAbfD3 on pNP-Araf and arabinan and comparative activity of XacAbf51 and TxAbfD3 on arabinoxylan.

From: The mechanism by which a distinguishing arabinofuranosidase can cope with internal di-substitutions in arabinoxylans

 

pNP-Araf

Arabinan

Arabinoxylana

Km (mM)

kcat (s−1)

kcat/Km (s−1 M−1)

Km (mg mL−1)

kcat (s−1)

kcat/Km (s−1 mg−1 mL)

v0/[E]t (s−1)

XacAbf51

0.19 ± 0.01

56.4 ± 0.8

3 × 105

20.4 ± 7.8

56.7 ± 6.4

2.8

9.1 ± 0.55

TxAbfD3

0.52 ± 0.06

154.2 ± 4.2

3 × 105

41 ± 9.5

27.15 ± 2.2

0.7

30.0 ± 1.31

  1. For comparative purposes, the assays were performed at 50 °C, pH 5.5, which is compatible with industrial processes of biomass saccharification. The Km and kcat could not be estimated for arabinoxylan because the maximum velocity is not reached at the highest possible concentration of the substrate
  2. aActivity measured using substrate at 10 mg mL−1
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