Fig. 2

Overall structures of OtCE15A (a) and SuCE15C (b). The catalytic triad (Ser-His-Glu) for each enzyme is shown as sticks. The inserted regions 1, 2, and 3 relative to the fungal CE15 enzymes are colored magenta, cyan, and green, respectively. Space filling representations of OtCE15A (c) and SuCE15C (d), highlighting the inserted regions relative to the methyl ester of 4-O-methyl glucuronoate substrate, generated from structural alignment with the co-crystallized structure of StGE2 (PDB: 4G4J)