Fig. 2

Glucan and xylan releases from Stage 1 (pretreatment; designated as “l”) and Stage 2 (7 days of C. thermocellum consolidated bioprocessing (CBP); designated as “s”) for a hydrothermal (180 °C), b hydrothermal (200 °C), c dilute acid, d dilute alkali, e co-solvent-enhanced lignocellulosic fractionation (CELF; 150 °C), and f CELF (140 °C) pretreatments. Pretreatments were performed at 10% solids loading (80 g switchgrass on a dry basis) with a total reaction mass of 800 g. CBP was performed at a 0.5 wt% glucan loading of all substrates with a working mass of 50 g