Fig. 5
Comparison of C. thermocellum consolidated bioprocessing (CBP) glucan solubilizations and fungal enzyme-mediated enzymatic hydrolysis (EH) glucan yields after 7 days of each biological operation for unpretreated switchgrass (SG) and solids prepared by hydrothermal (HT), dilute acid (DA), dilute alkali (Alk), and co-solvent-enhanced lignocellulosic fractionation (CELF) pretreatments at conditions that gave the highest total sugar release. Both CBP and EH were performed at 0.5 wt% glucan loading of all substrates with a working mass of 50 g. A 2% by volume inoculum was used for C. thermocellum CBP and 15 and 65 mg protein/g glucan loading of Accellerase® 1500 were used for EH