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Table 1 Media for thermophilic clostridia (MTC) for C. thermocellum CBP

From: Biomass augmentation through thermochemical pretreatments greatly enhances digestion of switchgrass by Clostridium thermocellum

Solution Component Reactor concentration (g/L) Stock concentration (g/L)
A MOPS (buffer) 10 100
B Citric acid potassium salt [C6H5K3O7·H2O] 2 50
Citric acid monohydrate 1.25 31.25
Na2SO4 1 25
KH2PO4 1 25
NaHCO3 2.5 62.5
C NH4Cl 1.5 75
D MgCl2·6H2O 1 50
CaCl2·2H2O 0.2 10
FeCl2·4H2O 0.2 5
l-Cysteine hydrochloride monohydrate 1 50
E Pyridoxamine dihydrochloride 0.02 1
p-Aminobenzoic acid 0.004 0.2
d-Biotin 0.002 0.1
Vitamin B12 0.002 0.1
  1. MOPS, 3-(N-morpholino)propanesulfonic acid (pKa 7.20)