Fig. 1

Schematic illustrating the GFP-fusion coupling FACS platform accelerating the heterologous gene expression process in T. reesei. a gfp gene was fused to the target gene and acted as the indicator for successful expression of heterologous genes and for spore separation through an FACS platform. Heterologous genes of unsuccessful expressions would be excluded following flow cytometry analysis, since no fluorescence was detected. For genes with successful expression, strains with gene expressed at expected levels can be rapidly obtained from a large candidate pool through cell sorting, as well as an additional confirmation of hyphae fluorescence on 96-well plate cultures. Time and numbers marked in the figure indicated the time and numbers of selected transformants in each procedure. b Cellular fatty acyl-CoA could be converted to fatty alcohol by functional fatty acyl-CoA reductase (FAR). The FAR viability was monitored using FAR fused with GFP with FAR expression levels being reflected by GFP fluorescence intensity