Fig. 2

Flow cytometry analysis of T. reesei cells harboring gfp-fused gene construct. Trpdi2 was selected as the homologous gene, which was confirmed as expressing functionally, for feasibility of flow cytometry analysis of T. reesei cells with gene–gfp-fusion construct. Both spores (a, b) and protoplast (c, d, generated from hyphae by enzymatic degradation) were utilized for the evaluations. pyr4-TU-6 strain served as the negative control in analyzing the fluorescence distribution of Trpdi2-gfp-TU-6 strain’s cell population. 100,000 or 30,000 cells were analyzed for spore or protoplast samples, respectively. Dashed and light-blue lines marking the same value of GFP-Log_Height were for direct comparisons of results from different panels. FSC forward scatter