Fig. 3

Flow cytometry analysis of T. reesei spores constitutively expressing gfp. GFP was driven by three strong promoters on originally constructed plasmids designed for convenient cloning (multiple cloning site) and multiple segment assembly (BioBrick assembly). These plasmids were co-transformed into TU-6 with P19-pyr4 for transformant’s spore collection. 100,000 cells were analyzed for each sample. Two batches of analysis were performed, with the same indications of the variation between samples. Dashed and light-blue lines marking the same value of GFP-Log_Height were for direct comparisons of results from different panels. FSC forward scatter