Fig. 4

Flow cytometry analysis of functional viability of fatty acyl-CoA reductases (FARs) in T. reesei. Spores harboring heterologous genes encoding FARs driven by the pdc promoter were analyzed for the evaluation of functional viable FARs in T. reesei. pyr4-TU-6 and Ppdc-gfp-TU-6 strains were used as the negative and positive controls, respectively. Gates marking the same value range were defined according to the fluorescence distribution of positive cells of Ppdc-gfp-TU-6 spores. Numbers indicate the mean ratio of gate-defined spore number to that of the whole-cell population, and standard deviation was calculated from triplicate analysis on transformant spore pools. 100,000 cells were analyzed for each sample. Two batches of experiments were performed, and significant differences were only observed between pyr4-TU-6 and Ppdc-Tafar1-gfp-TU-6 for FAR-expressing spores. FSC forward scatter