Table 1 Primers used in expression cassette construction in this study
From: A GFP-fusion coupling FACS platform for advancing the metabolic engineering of filamentous fungi
Primer | Sequence | Comments |
|---|---|---|
pyr4-cass-F | TCTAGATATCGGATCCATCCCGGCTTGCGCTTGGACCTCGC | pyr4 expression cassette |
pyr4-cass-R | ACTAGTAAGCTTCCTAGGATATGGAAGCTGATATCGTCGACAA | |
TrPDI2-egfp-1F | AATCTAGAATGGTCTTGATCAAGAGCCT | Trpdi2-gfp segment |
TrPDI2-egfp-1R | AACAGCTCCTCGCCCTTGCTCACCAGCTCGTCCTTCTGGTCCTCGT | |
TrPDI2-egfp-2F | ACGAGGACCAGAAGGACGAGCTGGTGAGCAAGGGCGAGGAGCTGTT | |
TrPDI2-egfp-2R | ATATGCATTTACTTGTACAGCTCGTCCAT | |
P19-Ppdc-F | CGGTACGCGCGGATCTTCCAGAGATTCTAGATATCCGCTAGCAGGACTTCCAGGGCTACTTGGCGCG | pdc promoter |
Ppdc-gfp-R | TGAACAGCTCCTCGCCCTTGCTCACGTCGACCTTGGGCCCCTGCAGAAGCTTCATGATTGTGCTGTAGCTGCGCTGCTTT | |
Ppdc-gfp-F | AAAGCAGCGCAGCTACAGCACAATCATGAAGCTTCTGCAGGGGCCCAAGGTCGACGTGAGCAAGGGCGAGGAGCTGTTCA | gfp |
gfp-Tcbh1-R | CACTGGCCGTAGTGAGACTGGGTAGCCATGGCTCGAGTTACTTGTACAGCTCGTCCATGCCG | |
gfp-Tcbh1-F | CGGCATGGACGAGCTGTACAAGTAACTCGAGCCATGGCTACCCAGTCTCACTACGGCCAGTG | cbh1 terminator |
Tcbh1-P19-R | GTTTGCACGCCTGCCGTTCGACGATACTAGTCCTAGGTGGCCTCGCAACGGACAAGTTGGTC | |
P19-Ptef1-F | CGGTACGCGCGGATCTTCCAGAGATTCTAGATATCCGCTAGCGGGACAGAATGTACAGTACTATACT | tef1 promoter |
Ptef1-gfp-R | TGAACAGCTCCTCGCCCTTGCTCACGTCGACCTTGGGCCCAAGCTTCATTTTGACGGTTTGTGTGATGTAGCGT | |
Ptef1-gfp-F | ACGCTACATCACACAAACCGTCAAAATGAAGCTTGGGCCCAAGGTCGACGTGAGCAAGGGCGAGGAGCTGTTCA | gfp |
gfp-Tegl1-R | ACGTGCACGTCTTGCACCCGCTGTACTGCAGCTCGAGGTCGACTTACTTGTACAGCTCGTCCATGCCG | |
gfp-Tegl1-F | CGGCATGGACGAGCTGTACAAGTAAGTCGACCTCGAGCTGCAGTACAGCGGGTGCAAGACGTGCACGT | egl1 terminator |
Tegl1-P19-R | GTTTGCACGCCTGCCGTTCGACGATACTAGTCCTAGGTGCATTTCAAGGGCGTTGCTGAGAG | |
P19-Ppki-F | CGGTACGCGCGGATCTTCCAGAGATTCTAGATATCCGCTAGCATAACGGTGAGACTAGCGGCCGGTC | pki promoter |
Ppki-gfp-R | TGAACAGCTCCTCGCCCTTGCTCACGTCGACCTTGGGCCCCTGCAGAAGCTTCATGGTTAAGAGGGTTCTTCCGGCTTCG | |
Ppki-gfp-F | CGAAGCCGGAAGAACCCTCTTAACCATGAAGCTTCTGCAGGGGCCCAAGGTCGACGTGAGCAAGGGCGAGGAGCTGTTCA | gfp |
gfp-Tcbh2-R | TGTTTGAAGCCCGGTCACGAAAGCCCCATGGCTCGAGTTACTTGTACAGCTCGTCCATGCCG | |
gfp-Tcbh2-F | CGGCATGGACGAGCTGTACAAGTAACTCGAGCCATGGGGCTTTCGTGACCGGGCTTCAAACA | cbh2 terminator |
Tcbh2-P19-R | GTTTGCACGCCTGCCGTTCGACGATACTAGTCCTAGGAAGAGGTGGAGTAATTGGAATCTAC | |
Atfar1-gfp-PstI-F | AAGGCCTGCAGATGGAATCCAATTGTGTTCAATTTC | Atfar1 |
Atfar1-gfp-SalI-R | AACCTGTCGACTTGTTTAAGCACATGGGTGATGAGG | |
Atfar6-gfp-PstI-F | AAGGTCTGCAGATGTGTTTTTATGGTGAGACGTCTT | Atfar6 |
Atfar6-gfp-SalI-R | AACCTGTCGACCTCAGTCTTCTTCTTAGAAAGAAAT | |
Tafar-gfp-HindIII-F | GGCCTAAGCTTATGGTGTCCATCCCCGAGTACTACG | Tafar1 |
Tafar-gfp-SalI-R | AACCTGTCGACGTATCGCATGGTGGAAGAGGCTCGA | Â |
Tef1-RT-F | GCTCTGCTCGCCTACACCCT | Real-time PCR |
Tef1-RT-R | TCTCCTTCTCCCAGCCCTTG | |
Tafar1-RT-F | CCGACCCAACACCTACAC | |
Tafar1-RT-R | GGTCCGTTAAAGTTGTCAATC |