Fig. 1

Expression of TnCelB in transgenic poplar lines. a Relative transcript level of TnCelB in leaf and developing xylem in transgenic events and WT measured by qPCR using reference genes (UBQ11 and EF1β) as the internal control; b MUCase activities of total soluble protein (TSP) from leaf tissue of transgenic events over a temperature range (25–100 °C); c MUCase activity of TSP extracted from leaf tissue and developing xylem of transgenic events and WT at 100 °C; and d CMCase activity of TSP extracted from leaf tissue and developing xylem of transgenic events and WT at 100 °C. The enzymatic activities of transgenic events were normalized to WT levels. Each value represents mean of technical triplicates of three individual plants per event with the standard error