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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Penicillium citrinum UFV1 β-glucosidases: purification, characterization, and application for biomass saccharification

Fig. 2

Chromatographic profile obtained after β-glucosidase purification. a Pellet from ammonium sulfate precipitation was resuspended and applied onto a phenyl sepharose column (AKTA PRIME, GE Healthcare Biosciences). Purified PcβGlu1—fractions 12–16. PcβGlu2—fractions 25–30. Activity was determined using pNPβGlu and MUβGlu substrates. b PcβGlu1 fractions from phenyl sepharose were pooled and applied onto a Superdex 200 column for molecular mass determination. c PcβGlu2 fractions from phenyl sepharose were pooled and applied onto a Superdex 200 column fractions for further purification and molecular mass determination. Purified PcβGlu2—fractions 29–32. Activity was measured with MUβGlu substrate

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