Table 4 Kinetic parameters of purified PcβGlu
Substrate | Km (mM) | kcat (s−1) | kcat/Km (s−1 mM−1) |
|---|---|---|---|
pNPβGlu | 0.28 ± 0.01 | 20.1 ± 0.4 | 71.9 ± 2.7 |
MUβGlu | 0.24 ± 0.01 | 13.1 ± 0.5 | 54.5 ± 2.5 |
Gentiobiose | 0.38 ± 0.04 | 9.1 ± 0.4 | 24.0 ± 2.6 |
Cellobiose | 1.7 ± 0.1 | 9.2 ± 0.4 | 5.4 ± 0.3 |
Cellotriose | 0.8 ± 0.1 | 11.0 ± 0.8 | 13.7 ± 1.8 |
Cellotetraose | 0.42 ± 0.05 | 11.8 ± 0.9 | 28.0 ± 3.5 |
Cellopentaose | 0.25 ± 0.02 | 11.1 ± 0.7 | 44.5 ± 3.8 |
Laminaribiose | 0.33 ± 0.04 | 9.8 ± 0.6 | 29.6 ± 3.7 |
Octyl-β-glucopyranoside | 0.54 ± 0.03 | 7.2 ± 0.3 | 13.4 ± 0.8 |
Laminarin | 0.09 ± 0.01 | 0.53 ± 0.04 | 5.9 ± 0.7 |