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Table 4 Kinetic parameters of purified PcβGlu

From: Penicillium citrinum UFV1 β-glucosidases: purification, characterization, and application for biomass saccharification

Substrate Km (mM) kcat (s−1) kcat/Km (s−1 mM−1)
pNPβGlu 0.28 ± 0.01 20.1 ± 0.4 71.9 ± 2.7
MUβGlu 0.24 ± 0.01 13.1 ± 0.5 54.5 ± 2.5
Gentiobiose 0.38 ± 0.04 9.1 ± 0.4 24.0 ± 2.6
Cellobiose 1.7 ± 0.1 9.2 ± 0.4 5.4 ± 0.3
Cellotriose 0.8 ± 0.1 11.0 ± 0.8 13.7 ± 1.8
Cellotetraose 0.42 ± 0.05 11.8 ± 0.9 28.0 ± 3.5
Cellopentaose 0.25 ± 0.02 11.1 ± 0.7 44.5 ± 3.8
Laminaribiose 0.33 ± 0.04 9.8 ± 0.6 29.6 ± 3.7
Octyl-β-glucopyranoside 0.54 ± 0.03 7.2 ± 0.3 13.4 ± 0.8
Laminarin 0.09 ± 0.01 0.53 ± 0.04 5.9 ± 0.7