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Table 5 Kinetic parameters of purified PcβGlu2

From: Penicillium citrinum UFV1 β-glucosidases: purification, characterization, and application for biomass saccharification

Substrate Km (mM) kcat (s−1) kcat/Km (s−1 mM−1) Ki (mM)
pNPβGlu 0.072 ± 0.007 1.36 ± 0.03 18.9 ± 1.9 2.4 ± 0.2
MUβGlu 0.014 ± 0.002 1.51 ± 0.06 105.2 ± 11.8 0.7 ± 0.1
Gentiobiose 0.62 ± 0.07 0.61 ± 0.02 1.0 ± 0.1
Cellobiose 0.64 ± 0.06 0.35 ± 0.01 0.55 ± 0.05
Cellotriose 0.31 ± 0.08 2.09 ± 0.04 6.7 ± 1.9 3.3 ± 1.2
Cellotetraose 0.28 ± 0.07 3.2 ± 0.4 11.2 ± 3.4 1.1 ± 0.3
Cellopentaose 0.07 ± 0.01 1.6 ± 0.3 22.3 ± 3.2 4.3 ± 0.8
Laminaribiose 0.42 ± 0.03 1.55 ± 0.06 3.7 ± 0.3 10.2 ± 1.3
Octyl-β-glucopyranoside 0.19 ± 0.03 0.26 ± 0.02 1.4 ± 0.2 4.2 ± 0.7
Laminarin 0.027 ± 0.001 1.20 ± 0.02 44.3 ± 1.8