Table 5 Kinetic parameters of purified PcβGlu2
Substrate | Km (mM) | kcat (s−1) | kcat/Km (s−1 mM−1) | Ki (mM) |
|---|---|---|---|---|
pNPβGlu | 0.072 ± 0.007 | 1.36 ± 0.03 | 18.9 ± 1.9 | 2.4 ± 0.2 |
MUβGlu | 0.014 ± 0.002 | 1.51 ± 0.06 | 105.2 ± 11.8 | 0.7 ± 0.1 |
Gentiobiose | 0.62 ± 0.07 | 0.61 ± 0.02 | 1.0 ± 0.1 | – |
Cellobiose | 0.64 ± 0.06 | 0.35 ± 0.01 | 0.55 ± 0.05 | – |
Cellotriose | 0.31 ± 0.08 | 2.09 ± 0.04 | 6.7 ± 1.9 | 3.3 ± 1.2 |
Cellotetraose | 0.28 ± 0.07 | 3.2 ± 0.4 | 11.2 ± 3.4 | 1.1 ± 0.3 |
Cellopentaose | 0.07 ± 0.01 | 1.6 ± 0.3 | 22.3 ± 3.2 | 4.3 ± 0.8 |
Laminaribiose | 0.42 ± 0.03 | 1.55 ± 0.06 | 3.7 ± 0.3 | 10.2 ± 1.3 |
Octyl-β-glucopyranoside | 0.19 ± 0.03 | 0.26 ± 0.02 | 1.4 ± 0.2 | 4.2 ± 0.7 |
Laminarin | 0.027 ± 0.001 | 1.20 ± 0.02 | 44.3 ± 1.8 | – |