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Table 3 CDS variations in SyBE_Sc2110M1 and SyBE_Sc2110M3 with respect to SyBE_Sc307001

From: Astaxanthin overproduction in yeast by strain engineering and new gene target uncovering

Chromosome

Gene

Position and mutate

Function

Specific to SyBE_Sc2110M1

 ChrIV

HKR1

1308011: V<->Aa

Zinc finger family member

1308047: V<->Aa

URC2

1482052: Y<->Ca

Putative transcription factor

 ChrVII

PDR1

470224: C<->Fa

Transcription factor

GTO1

797427: K<->Qa

Glutathione transferase

 ChrXII

UBR2

188380: Q<->Ha

Cytoplasmic ubiquitin-protein ligase

 ChrXIII

YMR317W

908196: W<->Sa

Hypothetical protein

908198: A<->Sa

YML084W

99616D: ATb

Hypothetical protein

 ChrXV

NDD1

1036169D: TTGc

Transcriptional activator

YOL166C

1109D: TAb

Hypothetical protein

 ChrXVI

RRG8

765953: N<->Ka

Hypothetical protein

Specific to SyBE_Sc2110M3

 ChrII

YBR012W-B

263145D: Cb

Retrotransposon TYA Gag

 ChrXII

YLR410W-B

944238: Q<->Ka

Retrotransposon TYA Gag and TYB Pol

Commonalities

 ChrI

FLO9

27080: V<->Ia

Mannose-binding lectin

25872: I<->Ma

26144: M<->La

 ChrII

YBL100W-A

30754: D<->Na

Retrotransposon TYA Gag

 ChrIII

TAF2

204436I: CTTCCTCTTCCb

RNA polymerase II transcription initiation

 ChrIV

YDR340W

1150913I: Gb

Hypothetical ORF

 ChrVIII

YHL050C

1748: N<->Sa

Hypothetical ORF

YHL041W

17546I: Tb

Hypothetical ORF

 ChrIX

CSS1

25586: D<->Ga

Hypothetical ORF

  

25418: D<->Ga

 ChrX

DAN4

715095: P<->Sa

Cell wall mannoprotein

715122: P<->Sa

YJR023C

470178: D: ATAc

Protein required for cell viability

 ChrXI

YKL225W

458I: Ab

Hypothetical ORF

 ChrXV

YOR192C-B

708819: A<->Ga

Retrotransposon TYA Gag

 ChrXIV

YNL338W

6637D: ACb

Hypothetical ORF

 ChrXVI

DPB2

896899: F<->Ya

DNA polymerase epsilon

  1. aMissense substitutions, numbers indicated the mutagenesis sites in chromosome, capital letters indicated the corresponding mutations on amino acids
  2. bFrame-shift mutations, numbers indicate the mutagenesis sites in chromosome, “D” is for deletion, “I” is for insertion, capital letters after “:” indicates the deleted or inserted base(s)
  3. cIn frame insertions/deletions, numbers indicate the mutagenesis sites in chromosome, “D” is for deletion, capital letters after “:” indicates the deleted or inserted base(s)