Fig. 1From: The hemicellulose-degrading enzyme system of the thermophilic bacterium Clostridium stercorarium: comparative characterisation and addition of new hemicellulolytic glycoside hydrolasesRelative activity of the secreted enzymes of C. stercorarium grown on oat spelt xylan (light grey) or filter paper (dark grey). Enzymatic activity was determined by DNSA-based assay. 5.0 mg/L extracellular enzymes, precipitated from the culture supernatant, were incubated with 0.5% (w/v) polysaccharide at pH 6.5 and 60 °C in 0.1 M MOPS reaction buffer overnight (16 h). Hydrolytic activity from oat spelt xylan medium on soluble wheat arabinoxylan was set to 100%. Tested polysaccharides with no activity were: arabinan, arabinogalactan (larch), curdlan, galactomannan (guar), gum arabic (acacia), inulin (dhalia tubers), laminarin, mannan, mannan (ivory nut), rhamnogalacturonan I, sinistrinBack to article page