Table 3 Hemicellulolytic activity of seventeen glycoside hydrolases on 29 different polysaccharides
Enzymes | Specific activity (U/mg) | |||||||
|---|---|---|---|---|---|---|---|---|
Arabinan | Arabinoxylan (wheat, soluble) | Arabinoxylan (wheat, insoluble) | 4-O-Methyl-glucuron-oxylan | Xylan (birch) | Xylan (oat spelt) | Xyloglucan | β-Glucan (barley) | |
Bga2B | (+) | – | – | – | – | – | 3.11 ± 0.04 | – |
Bxl3B | – | 0.25 ± 0.01 | + | 0.21 ± 0.01 | 0.28 ± 0.01 | 0.35 ± 0.02 | – | – |
Cel9Z | – | – | – | (+) | – | (+) | – | 159 ± 1 |
Xyn10E | – | 0.17 ± 0.01 | – | + | (+) | (+) | – | – |
Xyn10C | – | 134 ± 5 | 14.1 ± 0.8 | 91.3 ± 3.8 | 56.3 ± 2.0 | 86.6 ± 1.2 | – | 19.1 ± 0.1 |
Xyn10B | – | 597 ± 46 | 40.4 ± 3.7 | 483 ± 45 | 185 ± 20 | 679 ± 41 | – | 18.8 ± 1.6 |
Xyn10D | – | 6.55 ± 0.09 | – | + | 1.24 ± 0.04 | 6.17 ± 0.08 | – | (+) |
Xyn11A | – | 1030 ± 50 | 226 ± 47 | 936 ± 19 | 619 ± 7 | 937 ± 6 | – | – |
Man26A | – | – | – | – | – | – | + | – |
Bga35A | (+) | – | – | – | – | – | – | – |
Axh43A | + | 41.5 ± 0.8 | (+) | (+) | – | + | – | – |
Arf43A | – | (+) | – | (+) | (+) | (+) | – | – |
Arf43C | 0.82 ± 0.06 | – | – | – | – | – | – | – |
Abn43A | 79.5 ± 0.9 | – | – | – | – | – | – | – |
Arf51B | 2.01 ± 0.03 | 0.32 ± 0.01 | – | – | – | (+) | – | – |
Gal53A | + | – | – | – | – | – | – | – |
Xyn105F | – | 0.22 ± 0.01 | – | + | + | + | – | – |
Enzymes | Specific activity (U/mg) | |||||||
|---|---|---|---|---|---|---|---|---|
Lichenin | Galactan (potato) | Galactan (lupin) | Pectic galactan (potato) | Pectic galactan (lupin) | Mannan | Galactomannan (guar) | Glucomannan (konjac) | |
Bga2B | – | + | (+) | (+) | (+) | – | – | – |
Bxl3B | – | – | – | – | – | – | – | – |
Cel9Z | 136 ± 9 | – | – | – | – | – | – | 32.9 ± 1.0 |
Xyn10E | – | – | – | – | – | – | – | – |
Xyn10C | 7.85 ± 0.45 | – | – | – | (+) | – | – | + |
Xyn10B | 9.65 ± 0.65 | – | – | – | (+) | – | – | (+) |
Xyn10D | – | – | – | – | (+) | – | – | – |
Xyn11A | – | – | – | – | – | – | – | – |
Man26A | – | – | – | – | – | 39.9 ± 2.0 | 216 ± 5 | 144 ± 3 |
Bga35A | – | 2.17 ± 0.03 | 1.24 ± 0.08 | 1.08 ± 0.01 | 1.14 ± 0.02 | – | – | – |
Axh43A | – | – | – | – | (+) | – | – | – |
Arf43A | – | – | – | – | (+) | – | – | – |
Arf43C | – | – | 1.99 ± 0.20 | 6.50 ± 0.13 | 4.46 ± 0.09 | – | – | – |
Abn43A | – | – | 10.1 ± 0.3 | 21.3 ± 0.2 | 12.0 ± 2.1 | – | – | – |
Arf51B | – | – | – | (+) | – | – | – | – |
Gal53A | – | + | + | + | + | + | – | – |
Xyn105F | – | – | – | – | – | – | – | – |