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Table 3 Hemicellulolytic activity of seventeen glycoside hydrolases on 29 different polysaccharides

From: The hemicellulose-degrading enzyme system of the thermophilic bacterium Clostridium stercorarium: comparative characterisation and addition of new hemicellulolytic glycoside hydrolases

Enzymes Specific activity (U/mg)
Arabinan Arabinoxylan (wheat, soluble) Arabinoxylan (wheat, insoluble) 4-O-Methyl-glucuron-oxylan Xylan (birch) Xylan (oat spelt) Xyloglucan β-Glucan (barley)
Bga2B (+) 3.11 ± 0.04
Bxl3B 0.25 ± 0.01 + 0.21 ± 0.01 0.28 ± 0.01 0.35 ± 0.02
Cel9Z (+) (+) 159 ± 1
Xyn10E 0.17 ± 0.01 + (+) (+)
Xyn10C 134 ± 5 14.1 ± 0.8 91.3 ± 3.8 56.3 ± 2.0 86.6 ± 1.2 19.1 ± 0.1
Xyn10B 597 ± 46 40.4 ± 3.7 483 ± 45 185 ± 20 679 ± 41 18.8 ± 1.6
Xyn10D 6.55 ± 0.09 + 1.24 ± 0.04 6.17 ± 0.08 (+)
Xyn11A 1030 ± 50 226 ± 47 936 ± 19 619 ± 7 937 ± 6
Man26A +
Bga35A (+)
Axh43A + 41.5 ± 0.8 (+) (+) +
Arf43A (+) (+) (+) (+)
Arf43C 0.82 ± 0.06
Abn43A 79.5 ± 0.9
Arf51B 2.01 ± 0.03 0.32 ± 0.01 (+)
Gal53A +
Xyn105F 0.22 ± 0.01 + + +
Enzymes Specific activity (U/mg)
Lichenin Galactan (potato) Galactan (lupin) Pectic galactan (potato) Pectic galactan (lupin) Mannan Galactomannan (guar) Glucomannan (konjac)
Bga2B + (+) (+) (+)
Bxl3B
Cel9Z 136 ± 9 32.9 ± 1.0
Xyn10E
Xyn10C 7.85 ± 0.45 (+) +
Xyn10B 9.65 ± 0.65 (+) (+)
Xyn10D (+)
Xyn11A
Man26A 39.9 ± 2.0 216 ± 5 144 ± 3
Bga35A 2.17 ± 0.03 1.24 ± 0.08 1.08 ± 0.01 1.14 ± 0.02
Axh43A (+)
Arf43A (+)
Arf43C 1.99 ± 0.20 6.50 ± 0.13 4.46 ± 0.09
Abn43A 10.1 ± 0.3 21.3 ± 0.2 12.0 ± 2.1
Arf51B (+)
Gal53A + + + + +
Xyn105F
  1. Hydrolysis experiments were performed in 0.1 M MOPS reaction buffer with 0.05–24 mg/L enzyme at pH 6.0 (pH 6.5 for Abn43A) and the temperature optimum of each enzyme for incubation times from 30 min up to 2 h. The specific enzyme activity was determined using DNSA assay in triplicates. Further tested polysaccharides for which no activity could be determined were: arabinogalactan (larch), curdlan, gum arabic (acacia), inulin (dahlia tubers), laminarin, mannan (ivory nut), pachyman, polygalacturonic acid, pullulan, rhamnogalacturonan I, sinistrin, chitosan and Avicel. No activity (–), detectable but very low activity ((+)), activity overnight (+), specific activity (numbers)